Morph2 Server

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Contents

Introduction to Evolmorph

Evolmorph is a morphing server, similar to the Morph Server, but specifically designed for morphing between evolutionarily related proteins rather than different conformations of the same protein.

The server can be found here: http://evolmorph.gersteinlab.org/submit.html

Basically the steps are as follows:

  1. The user supplies two proteins in the form of pdb files.
  2. The two proteins are homogenized to have the same C-Alphas, using one of two methods (see below).
  3. The second protein is superimposed on the first so as to minimize the RMSD between the C-Alphas.
  4. Each atom from the first protein is linearly interpolated toward its position in the second, using the desired number of steps. Optionally, after each step, the protein is minimized using gromacs in order to create more realistic intermediate positions.
  5. Finally all the steps are combined into an animation, and the results stored in the evolmorph database.

Homogenization Methods

Evolmorph currently supports two methods of homogenizing the proteins so that they can be morphed:

Homology modeling

This method makes use of Modeller from the Sali lab http://www.salilab.org/modeller. Modeller creates a new protein that has the sequence of the first, and the structure of the second. Evolmorph then morphs the original first protein against this new protein.

Alignment homogenization

This method was created by Amit Oberai (Yale Gerstein Lab). In this method, the two proteins are aligned by residue. Gaps are removed, and mismatches replaced by simple, common residues, usually Glycine. In this way, two new proteins are created that have (nearly) the structure of the originals, but with a common set of residues.

Contact

For help with Evolmorph, contact robert.bjornson@yale.edu

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